5 specific examples of accomplishments in creative professional activity driven by clinical translational research or educational endeavors to improve professional practices:
1. Ngan B, Merico D, Marcus N, Kim V, Upton J, Bates A, Herbrick J, Nalpathamkalam T, Thiruvahindrapuram B, Cox P, Roifman C. Mutations in tetratricopeptide repeat domain 7A (TTC7A) are associated with combined immunodeficiency with dendriform lung ossification but no intestinal atresia. LymphoSign. 2014 May 1. 1(1) : 10-26, 10.14785/lpsn-2014-0002. Principal Author.
2. Shaikh F, Ngan BY, Alexander S, Grant R. Progressive transformation of germinal centers in children and adolescents: an intriguing cause of lymphadenopathy. Pediatr Blood Cancer. 2013 Jan;60(1):26-30. doi: 10.1002/pbc.24234. Epub 2012 Jun 15. (Trainee publication). Coauthor or Collaborator.
3. Sivagnanam M, Mueller JL, Lee H, Chen Z, Nelson SF, Turner D, Zlotkin SH, Pencharz PB, Ngan BY, Libiger O, Schork NJ, Lavine JE, Taylor S, Newbury RO, Kolodner RD, Hoffman HM. Rapid identification of EpCAM as the Gene for Congenital Tufting Enteropathy Using Two individuals. J. of Gastroenterology. 2008;135(2):429-37. Coauthor or Collaborator.
4. Ramphal R, Pappo A, Zielenska M, Grant R, Ngan B. Pediatric renal cell carcinoma: clinical, pathological and molecular abnormalities associated with the members of the MiT transcription factor family. Am J Clin Pathol. 2006;126:349-364 (Trainee publication). Senior Responsible Author.
5. Ngan BY, Verillo J-A, Lim MS. Lymph nodes, Bone marrow and Immunodeficiencies. In: Cohen MC and Scheimberg I, editor(s). Essentials of Surgical Pediatric Pathology. (UK): Cambridge University Press; 2014. p. 228-274. Co-Principal Author. I was invited to write this chapter by Drs M. Cohen and I. Scheimberg (the Chairpersons of the Pediatric Pathology Section of the European Society of Pathology in 2012).
I am currently an assistant professor holding an academic position as clinician teacher. My time commitment is distributed as follows: Clincial Activites 75%; Research activities 12%; Teaching activities 12% and administrative activites 1%.
My career objective has been focused on the generation of new knowledge in the recognition of previously unrecognized disease types, or the identification of previously unrecognized features within an existing disease which leads to an improved understanding of the mechanisms of pediatric diseases or malignancies. This would lead to an improvement of diagnosis and clinical management. This objective was accomplished through the selection of the case example(s) that I encountered from my clinical practice where they are subjected to extended analyses with immunohistochemical and molecular procedures to identify the pertinent pathobiological mechanism(s) that is/are associated with the cause of the disease.
The clinic-pathological focus of the publications listed in my CV are related to: 1. Defining a new disease entity by its association with unique clinical features, in conjunction with histopathologic, immunohistologic, cytogenetic and molecular abnormalities; 2. Contribute to the understanding of mechanism of disease and improve clinical management; 3. Identification of appropriate diagnostic tests for disease specific treatment; 4. High-lighting some of the disease features that are unique in children. As many of the childhood diseases I studied are uncommon, the clinical features are complex and the testing can be so complex that it exceeded the capacity of my research environment, many of these studies were accomplished by collaboration between members in other clinical departments within my hospital, nationally and internationally. 5. Dissemination of these new knowledge was effected via publication in peer reviewed journals and presentations in educational sessions hosted by pediatric pathology professional society educational meetings in the US, Europe and Asia, namely the Society of Pediatric Pathology (US), Annual Congress of Pathology of the European Society of Pathology and the Annual Meetings of the International academy of Pathology-Asia Division. I also contributed to several professional journals as guest reviewer of manuscripts.
Excellence in Research and CPA
The study of human diseases by microscopy is an observational science that had been in practice for more than a century. It requires not only superb visual skills but also the ability to observe and identify distinct morphological features so their significance in mechanism of diseases can then be tested. To accomplish this, newly discovered knowledge and analytical methods in basic biological sciences that can provide further information about the underlying biological processes are invaluable additions to the toolbox that can be applied to the study of human diseases. These are the fundamental approaches I adhere to in my pursuit of my creative professional activity in the field of human pathology.
Upon completion of my pathology training program, my specialization in studying human lymphoma started with the mentorship by one of the world’s leading expert in histomorphological classification of lymphomas, the late Dr. Ronald Dorfman. From him I learned the necessary observational skills that microscopy can ever provide. With his team of faculty that excelled in applying immune-labeling procedures to identify biological functions of human lymphocytes and the application of molecular biology to detect unique DNA abnormalities in cancer cells, it paved the way to extend and apply this approach to conduct clinical studies.
Career development and progress
My earlier clinical research focus was based on the initial discovery that a subtype of adult lymphoma that harbours an abnormal chromosomal translocation (t(14;18)) led to an abnormal expression of one of the translocated gene (BCL2) (CV publication #98 ). I received the Arthur Purdy Stout Prize from the Arthur Purdy Stout Society of Surgical Pathologists in 1989 for this discovery. Based on this abnormal feature that is highly associated with a common type of lymphoma (follicular lymphoma), two distinct diagnostic tests (one by immunohistochemistry and another by a highly sensitive molecular procedure (the polymerase chain reaction) could be designed to distinguish between malignant and neoplastic lympho-proliferative diseases (CV publication #98, 93). I founded the laboratory of molecular diagnosis in the Dept. of Pathology at the Sunnybrook Health Science Center that provide both state of the art molecular diagnostic tests (at that time) for lymphoid neoplasm, lymphoma research (Leukemia Foundation, Sunnybrook Foundation Grant, 1990; 100% to my lab) and epidemiological lymphoma research as a participant of a group grant with the Bayview Cancer Center at Sunnybrook Hospital. Together with the oncologists, we received support from MRC for this research. After 1997, my clinical research foci at the Hospital for Sick children were in 3 domains: Pathology of Pediatric Diseases and pediatric malignancies with specific focus in lymphoproliferative and immunodeficiency diseases. I serve as the pathology site group representative (for Toronto, at Sick Children’s Hospital) for the Children Oncology Group Clinical Research Project (a clinical trial research project funded by the US government) and I am also registered as an investigator of the Cancer Therapy Evaluation Program, under the Division of Cancer Treatment and Diagnosis of the National Cancer Institute of the United States of America for many years.
Career development in Childhood Diseases
My current career benefited from the clinical environment working at Canada’s unique academic center that provides comprehensive care to pediatric diseases where I can apply the same observation study skill sets coupled to the use of immunological and molecular analytical procedures to the discipline of pediatric pathology. The 5 examples below cited reflects the 4 goals I pursued in creative clinical activities that have been accomplished. These 4 goals are: 1. Defining a new disease entity by its association with unique clinical features, in conjunction with histopathologic, immunohistologic, cytogenetic and molecular abnormalities. 2. The findings arising from them contributed to the understanding of mechanism of disease and improve clinical management. 3. The findings provided a rationale for the application and identification of appropriate diagnostic tests for disease specific treatment. 4. The findings also highlighted diseases that are unique to children and in the last work cited, where the working knowledge of providing accurate diagnosis of lymphoma in children is presented as a book chapter as a venue of knowledge dissemination to other practicing professionals.
Clinical diagnostic pathology laboratory is supported by hitopathology, immunohistochemistry, ultra-structural pathology, molecular pathology with long time excellent collaborative support from Cytogenetics and Clinical Immunology. Research is clincal research focused to foster the development of innovative diagnostics, clincial patient care and training of next generation of pathologists through the application of new analytical technologies to elucidate the mechansims that involves the pathogenesis diseases, and apply the knowledge gained to improve the diagnsosis of childhood diseases.
Can only accept trainees with external funding .
Selected list of publications
1.Ceppi F, Pope E, Ngan B, Abla O. Primary cutaneous lymphomas in children and adolescents. Pediatric Blood and Cancer. 2016 Apr 28. In Press.
2. Ngan B, Merico D, Marcus N, Kim V, Upton J, Bates A, Herbrick J, Nalpathamkalam T, Thiruvahindrapuram B, Cox P, Roifman C. Mutations in tetratricopeptide repeat domain 7A (TTC7A) are associated with combined immunodeficiency with dendriform lung ossification but no intestinal atresia. LymphoSign. 2014 May 1. 1(1) : 10-26, 10.14785/lpsn-2014-0002.
3. Sharfe N, Nahum A, Newell A, Dadi H, Ngan B, Pereira SL, Herbrick JA, Roifman CM. Fatal combined immunodeficiency associated with heterozygous mutation in STAT1. J Allergy Clin Immunol. 2014 Mar;133 (3):807-17. 2014 Mar: 133(3):807-17. doi: 10.1016/j.jaci.2013.09.032. Epub 2013 Nov 13.
4. Kirwan C, Ngan BY, Halliday W, Alexander S, Ali A. Primary conjuctival analplastic large cell lymphoma in a child. J Amer Assoc Ped Ophthalmology Strabismus. 2013 Aug;17(4):437-439
5. Malowany JI, Merritt NH, Chan NG, Ngan BY. Nested stromal epithelial tumor of the liver in Beckwith-Wiedemann syndrome. Pediatr Dev Pathol. 2013 Jul;16(4):312-317. 2013 Apr 9 [Epub ahead of print]
6. Shaikh F, Ngan BY, Alexander S, Grant R. Progressive transformation of germinal centers in children and adolescents: an intriguing cause of lymphadenopathy. Pediatr Blood Cancer. 2013 Jan;60(1):26-30. doi: 10.1002/pbc.24234. Epub 2012 Jun 15
7. Al Mahmoud R, Weitzman S, Schechter-Finkelstein T, Ngan B, Abdelhaleem M, Alexander S. Peripheral T-cell lymphoma in children and adolescents: A single-institution experience.
8.Naithani R, Ngan BY, Roifman C, Crump M, Baruchel S and Abla O. Thymic mucosa-associated lymphoid tissue lymphoma in an adolescent girl. J Pediatr Hematol Oncol. 2012 Oct;34(7):552-555.
9. Newell A, Dadi H, Goldberg R, Ngan BY, Grunebaum E, Roifman CM. Diffuse large B-cell lymphoma as presenting feature of Zap-70 deficiency. J Allergy Clin Immunol. 2011 Feb;127(2):517-520.
10. Manlhiot C, Pollock-Barziv SM, Holmes C, Weitzman S, Allen U, Clarizia NA, Ngan BY, McCrindle BW, Dipchand AI. Post-transplant lymphoproliferative disorder in pediatric heart transplant recipients. J Heart Lung Transplant. 2010;29(6):648-57
11. Pope E, Weitzman S, Ngan BY, Walsh S., Morel K, Williams J, Stein S, Garzon M, Knobler E, Lieber C, Turchan K, Wargon O, Tsuchiya A. Mycosis Fungoides in the pediatric population: Report from an International Childhood Registry of Cutaneous Lymphoma. Journal of Cutaneous Medicine & Surgery. 2010;14(1):1-6.
12.Chong AL, Ngan BY, Weitzman S, Abla O. Anaplastic large cell lymphoma of the ovary in a pediatric patient. J Pediatr Hematol Oncol. 2009;31(9):702-4
13. Sivagnanam M, Mueller JL, Lee H, Chen Z, Nelson SF, Turner D, Zlotkin SH, Pencharz PB, Ngan BY, Libiger O, Schork NJ, Lavine JE, Taylor S, Newbury RO, Kolodner RD, Hoffman HM. Rapid identification of EpCAM as the Gene for Congenital Tufting Enteropathy Using Two individuals. J. of Gastroenterology. 2008;135(2):429-37.
14. Ngan BY, Fort V, Campisi P. Molecular angiogenic signaling in angiofibromas after embolization: implications for thereapy. Archives of Otolaryngology Head Neck Surgery. 2008;134(11):1170-6.
15.Husain M, Grunebaum E, Naqvi A, Atkinson A, Ngan BY, Aiuti A, Roifman CM. Burkitt’s lymphoma in a patient with adenosine deaminase deficiency-severe combined immunodeficiency treated with polyethylene glycol-adenosine deaminase. J Pediatr. 2007;151(1):93-5
16.Ramphal R, Pappo A, Zielenska M, Grant R, Ngan B. Pediatric renal cell carcinoma: clinical, pathological and molecular abnormalities associated with the members of the MiT transcription factor family. Am J Clin Pathol. 2006;126:349-364
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