Paul Krzyzanowski

Dr. Paul Krzyzanowski is the Director of the Genome Research Platform, part of the Genomics Program at the Ontario Institute for Cancer Research. In this capacity, his group delivers state of the art genomics services for researchers across Ontario and Canada.

Since joining the program in 2016, Dr. Krzyzanowski has led the platform through several stages of growth and transformation towards a goal of laboratory accreditation by the College of American Pathologists, slated to be granted in early 2021.

In addition to this role, Dr. Krzyzanowski’s academic interests lie in the development of novel genome technologies and statistical techniques to analyze genomic data.

He is a co-inventor of SimSen-Seq, a patented technique used for ultra-sensitive mutation detection in cancer monitoring settings published in Nature Protocols.

Prior to this role, Dr. Krzyzanowski was a CIHR-funded Postdoctoral Fellow in the Informatics and Biocomputing Program at OICR. He also holds a MBA from the Rotman School of Management, University of Toronto, a PhD in Molecular Medicine from the University of Ottawa, and a BSc(Honours) from the University of Toronto.

Follow Paul Krzyzanowski on Twitter: @pmkrzyzanowski

Research Synopsis

My overall research objectives relate to extend the capabilities of state of the art ‘omics technologies to support the translation of ‘omics techniques and methods to enable new applications in basic research, pathology, and oncology.

Recent Publications

Selected publications

Simple multiplexed PCR-based barcoding of DNA for ultrasensitive mutation detection by next-generation sequencing. Ståhlberg A, Krzyzanowski PM, Egyud M, Filges S, Stein L, Godfrey TE. Nature Protocols. 2017 Apr;12(4):664-682.

GATA6 expression distinguished classical and basal-like subtypes in advanced pancreatic cancer. G.M. O’Kane, B. Grünwald, GH Jang, M. Masoomian, S. Picardo, R.C. Grant, R.E. Denroche, A. Zhang, Y. Wang, B. Lam, P. Krzyzanowski, I. Lungu, J.M.S. Bartlett, M. Peralta, F. Vyas, R. Khokha, J.J. Biagi, D. Chadwick, S. Ramotar, S. Hutchinson, A. Dodd, J.M. Wilson, F. Notta, G. Zogopoulos, S. Gallinger, J. J. Knox, , S.E. Fischer. Clinical Cancer Research, 2020. 

Combined EsophaCap cytology and MUC2 immunohistochemistry for screening of intestinal metaplasia, dysplasia and carcinoma. Zhou Z, Kalatskaya I, Russell D, Marcon N, Cirocco M, Krzyzanowski PM, Streutker C, Liang H, Litle VR, Godfrey TE, Stein L. Clinical and Experimental Gastroenterology. 15 May 2019 Volume 2019:12 Pages 219—229. 

Regional perturbation of gene transcription is associated with intrachromosomal rearrangements and gene fusion transcripts in high grade ovarian cancer. Paul M Krzyzanowski, Fabrice Sircoulomb, Fouad Yousif, Josee Normand, Kyle Francis, Fernando Suarez, Timothy Beck, John D McPherson, Lincoln D Stein, Robert K Rottapel. Scientific Reports. March 5, 2019. 

Impact of Chemical-Induced Mutational Load Increase on Immune Checkpoint Therapy in Poorly Responsive Murine Tumors. Kuczynski EA, Krueger J, Chow A, Xu P, Man S, Sundaravadanam Y, Miller JK, Krzyzanowski PM, Kerbel RS. Mol Cancer Ther. 2018 Apr;17(4):869-882. doi: 10.1158/1535-7163.MCT-17-1091.

Plasma circulating tumor DNA as a potential tool for disease monitoring in head and neck cancer. Matthew Egyud, Praveen Sridhar, Anand Devaiah, Emiko Yamada, Stefanie Saunders, Anders Ståhlberg, Stefan Filges, Paul Krzyzanowski, Irina Kalatskaya, Wei Jiao, Lincoln Stein, Scharukh Jalisi, Tony Godfrey. Head & Neck. November 19, 2018. doi:10.1002/hed.25563

Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing. Anders Ståhlberg, Paul M. Krzyzanowski, Jennifer B. Jackson, Matthew Egyud, Lincoln Stein and Tony E. Godfrey. Nucleic Acids Research, 2016 Jun 20;44(11):e105. doi: 10.1093/nar/gkw224. Epub 2016 Apr 7.

Appointments

Institute Scientist, Ontario Institute for Cancer Research

Honours and Awards

Name: Dean’s List and Bregman Scholar, Rotman School of Management, University of Toronto (2017)
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Name: Arbor Award, University of Toronto (2015)
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Name: CIHR/Canadian Association of Gastroenterology Postdoctoral Fellow (2014)
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