LMP1203H: Basic principles of analytical clinical biochemistry

Who can attend

The course is open to medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.

Graduate students need permission from the supervisor and must be registered in the LMP graduate program to attend.


No specific course is required.

Course description

This course introduces current analytical techniques as applied in clinical biochemistry laboratories. We will cover the theoretical aspects of each technique, the limitations, and common applications. The course also introduces analytical and clinical evaluation of laboratory methods.

The course will be run as tutorials/lectures for medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.

Meetings will take place on Wednesday mornings (9:00-12:00 noon). Alternative meeting times and places may be arranged with individual lecturers if deemed necessary.

Course coordinator

Davor Brinc, PhD, FCACB

lmp.grad@utoronto.ca for administrative queries.

Timings and location

This course is offered every year in the Fall session.

Wednesdays, (9:00 a.m. - 12:00 noon)

Location: MS 3290 (note that selected lectures will be delivered via Zoom; we will email details for those in advance)


The students are responsible for the material provided in the lectures.

Optional readings

Selected chapters from Nader Rifai (editor). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.

Supplementary readings can also be provided by each lecturer

Evaluation methods

Final end-of-course exam.

Take-home assignments to be submitted on the day of the final exam may be used if deemed appropriate by the individual faculty member.


Lectures will be in person unless stated via Zoom.

Date Topic Lecturer/instructor
September 8, 2021 Analytical method evaluation Dr. Davor Brinc (UHN)
September 15, 2021

Clinical method evaluation

Via Zoom

Dr. Dorothy Truong, LifeLabs


September 22, 2021

General lab techniques: centrifugation, water purification, osmometry, radioactivity, viscometry, dialysis, freeze drying, buffers

Dr. Felix Leung 
September 29, 2021

Enzymatic techniques and chemistry analyzers

Dr. Felix Leung
October 6, 2021

Spectrophotometry: reflectance, absorbance, transmittance, turbidimetry, nephelometry, flameemission/absorption, IR, NMR, fluorescence

Via Zoom

Dr. Maria Pasic

Dr. Sarah Delaney, Unity Health Toronto (Sarah.Delaney@

October 13, 2021


Via Zoom

Dr. Saranya Arnoldo
October 20, 2021

Electrochemistry applications in a clinical chemistry laboratory

Via Zoom

Dr. Mohamed Abouelhassan

October 27, 2021

Immunoassays I: properties of antibodies and antigens; Ag-Ab interactions; Ab production

Dr. Paul Yip
November 3, 2021 Immunoassays II: configurations, labels, and interferences Dr. Paul Yip
November 10, 2021


Via Zoom

Dr. Daniel Beriault
November 17, 2021

Mass Spectrometry 

Dr. Andrea Bozovic
November 24, 2021

Point-of-care testing

Dr. Davor Brinc
December 1, 2021

Molecular diagnostics

Via Zoom

Dr. Cuihong Wei
December 8, 2021

Ultracentrifugation and lipid analyses

Via Zoom

Dr. Dana Bailey, Dynacare (BaileyD@dynacare.ca)

December 15, 2021 Final Examination  Dr. Davor Brinc