LMP1203H: Basic principles of analytical clinical biochemistry
Who can attend
The course is open to medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.
Graduate students need permission from the supervisor and must be registered in the LMP graduate program to attend.
Prerequisites
No specific course is required.
Course description
This course introduces current analytical techniques as applied in clinical biochemistry laboratories. We will cover the theoretical aspects of each technique, the limitations, and common applications. The course also introduces analytical and clinical evaluation of laboratory methods.
The course will be run as tutorials/lectures for medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.
Meetings will take place on Wednesday mornings (9:00-12:00 noon). Alternative meeting times and places may be arranged with individual lecturers if deemed necessary.
Course coordinator
lmp.grad@utoronto.ca for administrative queries.
Timings and location
This course is offered every year in the Fall session.
Wednesdays, 9 am - 12 noon
Location: TBA
Readings
Students are responsible for the material provided in the lectures.
Optional readings
Selected chapters from Nader Rifai (editor). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.
Supplementary readings can also be provided by each lecturer.
Evaluation methods
Final end-of-course exam.
Take-home assignments to be submitted on the day of the final exam may be used if deemed appropriate by the individual faculty member.
Schedule
Lectures will be in person unless stated via Zoom.
Date |
Topic |
Lecturer/instructor |
---|---|---|
September 8, 2021 |
Analytical method evaluation |
Dr. Davor Brinc (UHN) |
September 15, 2021 |
Clinical method evaluation Via Zoom |
Dr. Dorothy Truong, LifeLabs (dorothy.truong@lifelabs.com) |
September 22, 2021 |
General lab techniques: centrifugation, water purification, osmometry, radioactivity, viscometry, dialysis, freeze drying, buffers |
|
September 29, 2021 |
Enzymatic techniques and chemistry analyzers |
|
October 6, 2021 |
Spectrophotometry: reflectance, absorbance, transmittance, turbidimetry, nephelometry, flameemission/absorption, IR, NMR, fluorescence. Via Zoom |
Dr. Sarah Delaney, Unity Health Toronto (Sarah.Delaney@ |
October 13, 2021 |
Electrophoresis Via Zoom |
|
October 20, 2021 |
Electrochemistry applications in a clinical chemistry laboratory Via Zoom |
|
October 27, 2021 |
Immunoassays I: properties of antibodies and antigens; Ag-Ab interactions; Ab production |
|
November 3, 2021 |
Immunoassays II: configurations, labels, and interferences |
|
November 10, 2021 |
Chromatography Via Zoom |
|
November 17, 2021 |
Mass Spectrometry |
|
November 24, 2021 |
Point-of-care testing |
|
December 1, 2021 |
Molecular diagnostics Via Zoom |
|
December 8, 2021 |
Ultracentrifugation and lipid analyses Via Zoom |
Dr. Dana Bailey, Dynacare (BaileyD@dynacare.ca) |
December 15, 2021 |
Final exam |