LMP1203H: Basic principles of analytical clinical biochemistry

Who can attend

The course is open to medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.

Graduate students need permission from the supervisor and must be registered in the LMP graduate program to attend.

Prerequisites

No specific course is required.

Course description

This course introduces current analytical techniques as applied in clinical biochemistry laboratories. We will cover the theoretical aspects of each technique, the limitations, and common applications. The course also introduces analytical and clinical evaluation of laboratory methods.

The course will be run as tutorials/lectures for medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.

Meetings will take place on Wednesday mornings (9:00-12:00 noon). Alternative meeting times and places may be arranged with individual lecturers if deemed necessary.

Course coordinator

Dr. Davor Brinc

davor.brinc@uhn.ca

lmp.grad@utoronto.ca for administrative queries.

Timings and location

This course is offered every year in the Fall session.

Wednesdays, 9 am - 12 noon

Location: MS 3290 (note that selected lectures will be delivered via Zoom; we will email details for those in advance)

Readings

Students are responsible for the material provided in the lectures.

Optional readings

Selected chapters from Nader Rifai (editor). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.

Supplementary readings can also be provided by each lecturer.

Evaluation methods

Final end-of-course exam.

Take-home assignments to be submitted on the day of the final exam may be used if deemed appropriate by the individual faculty member.

Schedule

Lectures will be in person unless stated via Zoom.

Date

Topic

Lecturer/instructor

September 8, 2021

Analytical method evaluation

Dr. Davor Brinc (UHN)

September 15, 2021

Clinical method evaluation

Via Zoom

Dr. Dorothy Truong, LifeLabs

(dorothy.truong@lifelabs.com) 

September 22, 2021

General lab techniques: centrifugation, water purification, osmometry, radioactivity, viscometry, dialysis, freeze drying, buffers

Dr. Felix Leung 

September 29, 2021

Enzymatic techniques and chemistry analyzers

Dr. Felix Leung

October 6, 2021

Spectrophotometry: reflectance, absorbance, transmittance, turbidimetry, nephelometry, flameemission/absorption, IR, NMR, fluorescence.

Via Zoom

Dr. Maria Pasic

Dr. Sarah Delaney, Unity Health Toronto

(Sarah.Delaney@
unityhealth.to) 

October 13, 2021

Electrophoresis

Via Zoom

Dr. Saranya Arnoldo

October 20, 2021

Electrochemistry applications in a clinical chemistry laboratory

Via Zoom

Dr. Mohamed Abouelhassan

October 27, 2021

Immunoassays I: properties of antibodies and antigens; Ag-Ab interactions; Ab production

Dr. Paul Yip

November 3, 2021

Immunoassays II: configurations, labels, and interferences

Dr. Paul Yip

November 10, 2021

Chromatography

Via Zoom

Dr. Daniel Beriault

November 17, 2021

Mass Spectrometry 

Dr. Andrea Bozovic

November 24, 2021

Point-of-care testing

Dr. Davor Brinc

December 1, 2021

Molecular diagnostics

Via Zoom

Dr. Cuihong Wei

December 8, 2021

Ultracentrifugation and lipid analyses

Via Zoom

Dr. Dana Bailey, Dynacare

(BaileyD@dynacare.ca)

December 15, 2021

Final exam

Dr. Davor Brinc