LMP1203H: Basic principles of analytical clinical biochemistry
Who can attend
The course is open to medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.
Graduate students need permission from the supervisor and must be registered in the LMP graduate program to attend.
Prerequisites
Undergraduate course in biochemistry and/or molecular biology is recommended.
Course description
This course introduces current analytical techniques as applied in clinical biochemistry laboratories. We will cover the theoretical aspects of each technique, the limitations, and common applications. The course also introduces analytical and clinical evaluation of laboratory methods.
The course will be run as tutorials/lectures for medical biochemistry residents, postdoctoral diploma in clinical chemistry fellows, and graduate students.
Meetings will take place on Wednesday mornings (9:00-12:00 noon). Alternative meeting times and places may be arranged with individual lecturers if deemed necessary.
Course coordinator
lmp.grad@utoronto.ca for administrative queries.
Timings and location
This course is offered every year in the Fall session.
Wednesdays, 9 am - 12 noon
Location: MSB 3290
Readings
Students are responsible for the material provided in the lectures.
Optional readings
Selected chapters from Nader Rifai (editor). Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.
Supplementary readings can also be provided by each lecturer.
Evaluation methods
Final end-of-course exam (lectures 3,5-10,14): 60%
Take-home assignments for selected lectures (indicated in the table below): 40%
Schedule
Lectures will be in person unless stated via Zoom. Zoom details will be provided by email.
|
Date |
Topic |
Lecturer/instructor |
|---|---|---|
|
September 7, 2022 |
Introduction to analytical clinical biochemistry Analytical method evaluation Take-home assignment |
Dr. Davor Brinc (UHN) |
|
September 14, 2022 |
Clinical method evaluation Via Zoom Take-home assignment |
Dr. Dorothy Truong, LifeLabs (dorothy.truong@lifelabs.com) |
|
September 21, 2022 |
Chromatography Via Zoom |
|
|
September 28, 2022 |
Spectrophotometry: reflectance, absorbance, transmittance, turbidimetry, nephelometry, flame emission/absorption, IR, NMR, fluorescence Take-home assignment |
Dr. Uvaraj Uddayasankar LifeLabs (uvaraj.uddayasankar@lifelabs.com) |
|
October 7, 2022 |
General lab techniques: centrifugation, water purification, osmometry, radioactivity, viscometry, dialysis, freeze drying, buffers Via Zoom |
|
|
October 12, 2022 |
Enzymatic techniques and chemistry analyzers |
|
|
October 19, 2022 |
Electrochemistry applications in a clinical chemistry laboratory Via Zoom |
|
|
October 26, 2022 |
Immunoassays I: properties of antibodies and antigens; Ag-Ab interactions; Ab production |
|
|
November 2, 2022 |
Immunoassays II: configurations, labels, and interferences |
|
|
November 9, 2022 |
Electrophoresis Via Zoom |
Dr. Saranya Arnoldo William Osler (saranya.arnoldo@williamoslerhs.ca) |
|
November 16, 2022 |
Mass Spectrometry Take-home assignment |
|
|
November 23, 2022 |
Point-of-care testing Take-home assignment |
|
|
November 30, 2022 |
Molecular diagnostics Via Zoom Take-home assignment |
|
|
December 7, 2021 |
Ultracentrifugation and lipid analyses |
Dr. Dana Bailey, Dynacare (BaileyD@dynacare.ca) |
|
December 14, 2022 |
Final exam |