LMP1207H: Mass spectrometry, proteomics and their clinical applications

Who can attend

You must be registered in a graduate program to attend this course.

This course is open to all graduate students at the University of Toronto, provided you have pre-approval from your department and the course coordinators.

Course description

You will learn the fundamentals of mass spectrometry and its use with biochemical techniques to provide analyses of proteins and small molecules.

You will also study the interface with bioinformatics and other -omics technologies.

The second half of the course is designed to bridge the gap between basic and translational sciences and will examine the research and clinical applications of mass spectrometry.

Course coordinators

Dr. Vathany Kulasingam


Dr. Lusia Sepiashvili


lmp.grad@utoronto.ca for administrative queries.

Timings and location

This course is offered every even year in the Fall term (i.e. 2020, 2022 etc).

Wednesdays 10 am – 12 pm

Location: HS 705, Health Sciences Building 155 College Street, Toronto

Evaluation methods

Two written examinations:

  • Mid-term - 30%
  • Final exam - 30%

Presentation of a journal article - 20%

Take-home assignment - 20%





September 7, 2022

Introduction to mass spectrometry and proteomics

Dr. Vathany Kulasingam

September 14, 2022

Basic principles of mass spectrometry

Dr. Andrea Bozovic

September 21, 2022

Fundamentals of proteomics

Dr. Lusia Sepiashvili

September 28, 2022

Bioinformatics: Protein Identification and Targeted Proteomics

Dr. Vathany Kulasingam

October 5, 2022

Integration of proteomics with other -omics technologies

Dr. Voisin

October 12, 2022

Post-translational modifications and Protein-protein interactions

Dr. Kuzmanov

October 19, 2022

Mid-Term Exam


October 26, 2022

Analytical workflows for sub-proteome analyses

Dr. Lusia Sepiashvili

November 2, 2022

Mass spectrometry for metabolic and molecular genetics

Dr. Kyriakopoulou

November 9, 2022

Mass Spectrometry Imaging

Dr. Zarrine-Afsar

November 16, 2022

Clinical microbiology and toxicology

Dr. Poutanen

Dr. Stefan

November 23, 2022

Global Metabolomics

Dr. Nikita Looby

November 30, 2022

Emerging applications of Proteomics and MS

Dr. Kulasingam

Dr. Sepiashvili

December 7, 2022

Final Exam